Warming Kit 102

Advantages of Cryotec Warming Kit

This box contains all the tools and solutions you need to thaw oocytes and embryos
The protocol can be used for both oocytes and embryos

Cryotec Warming Plate

A well for the thawing stage designed for rapid warming
Waste-fluid drains on the side for efficient operations
Hemispherical shape prevents sudden changes in osmotic pressure
An indicator (3 mm) is installed on the lid of the warming plate to precisely measure the amount of solutions
Round-bottom eliminates shadows caused by light refraction

Cryotec Warming Solutions

Safe and long-lasting due to solutions being free from Serum and SSS
Low impact on cells due to the use of endotoxin-free trehalose and a low concentration of CPA
Preventing damage to oocytes and embryos due to the specific gravity of VS
Hydroxypropyl cellulose (HPC) increases viscosity and slows down the movement of oocytes and embryos

Specification

For 1 application
1.8 ml Thawing Solution (TS) x1
0.5 ml Diluent Solution (DS) x1
1.0 ml Washing Solution (WS) x1
Warming Plate x1

Shelf-life: 1 year at 2～8℃

Quality control

Our products are ISO 13485 certified
We only ship lots that passed POA test

Note

Cryotec solutions are available only in select countries, please contact globalsales@reprolife.jp to inquire

Protocol

Warming

A major issue when thawing has been the fact that sudden changes in osmotic shock lead to over-expansion (hyper-hydration) in the oocytes and embryos, causing damage to the cells and cytoskeletons. Cryotec preservation is a method which minimizes osmotic shock in the various solutions while adding HPC, thereby increasing viscosity and achieving extremely slow and safe dilution.